Hydrogen Peroxide Concentration 0% 4% 8% 10% 12% 16% 20% Time Taken Test 1 47. It could be repeated more times to help get rid of any anomalies. From these results a graph can be plotted with concentration on the x-axis and time taken on the y-axis. Catalase is a substrate, O2 and H2O are substrates, and H2O2 is an enzyme. The structure is shown below. Repeat all the tests at least three times so that an average can be obtained.
The optimum rate of reaction ,theoretically, is when all the active sites are being used but in reality this is never reached because not all the active sites are being used at the time. Hydrogen Peroxide is the poisonous by-product of metabolism. The gloves protect you from contamination it 's also important to wash your hands after each interaction with a patient to stop the spread of contamination. Introduction The fact that enzymes function as biological catalysts has a significant value in millions of living organisms including human beings. Gas Syringe Metal Stand Yeast Catalase Hydrogen Peroxide Test Tubes Beakers Test Tube Rack Stop Watch Pipette Pipette Filler Tap Water Plan 1. Hydrogen is found in group 1 and period 1 on the periodic table. The rate of enzyme controlled reactions is dependent on temperature, pH, and concentration of the substrate and enzyme.
The time taken to fit into and leave the active site is the limiting factor in the rate of reaction. Enzymes can be describe… 1344 Words 6 Pages The purpose of this experiment is to measure the rate of catalase enzyme activity under different temperature conditions. They are used to speed up specific reactions in the cells. Every 10, 30, 60, 90, 120, 180, and 360 second H2O2 was killed with acid. Our findings illustrate that the rate of enzyme activity is only influenced by substrate concentration at low level of substrate concentration, and as substrate concentration increases, the rate becomes independent of the substrate concentration and proceeds at a constant state.
Page 2 Concept Diagram…………………………………………………Page 3 Literature Reviews………………………………………………. Six labeled cups were set out according to their times and 10 mL of H2O2 were added to each cup. If you double the concentration of Hydrogen Peroxide then the rate of reaction doubles as well. Hypothesis The enzyme catalase, under optimum conditions, effectively speeds the decomposition of hydrogen peroxide. I've taken college-level chemistry, so I remem … ber how to do this. They are used to speed up specific reactions in the cells. Results When I carried out the experiment I got these results.
Then, 1 mL of fresh catalase solution was added and the reaction was observed and recorded. Extracts of this project must not be included in any projects that you submit for marking. Using the appropriate tool cut the potatoes into cylinder form measuring the cylinders with a ruler. If on the other hand, those are just regular variables, then there's really nothing wrong with it, other than the facts th … at it can be simplified, and that there is no equality given ie. After 16% the increase in the rate of reaction slowed down. Several experiments will be conducted using various combinations of concentrations of the reactant hydrogen peroxide and a catalyst potassium iodide , as well as one pairing in which a change in temperature is the only variable. Introduction This is an A-level biology project.
The only anomalies are the results at 8% and 10%. Prediction: I predict that the higher the amount of Hydrogen Peroxide I use then the faster the displacement reaction will be. What I know is that this type of reaction should be catabolism and not anabolism as you said. The strong nuclear force and the residual strong force hold the nucleus together. H2 g + O2 g H2O2 l Calculate the enthalpy change using the following data.
The type of reaction involved is known as a catabolic reaction i. The table below shows what amounts of Hydrogen Peroxide and water are needed to make the solutions. Doing this could lead to being disqualified from all the subjects that you are taking. The enzyme catalyzes catechol to produce benzoquinone, which is one of the causes of browning in many fruits and vegetables. The higher the concentration of. Hypothesis: Enzyme activity can be changed by substrate concentration and enzyme.
Assume the density of oxygen is 1. Hydrogen Peroxide Concentration 0% 4% 8% 10% 12% 16% 20% Time Taken Test 1 secs 47. The theoretical maximum rate of reaction is when all the sites are being used but in reality this theoretical maximum is never reached due to the fact that not all the active sites are being used all the time. They are used to speed up specific reactions in the cells. Nobody likes paying more money for gas and yet nobody is willing to do anything about it.
This is shown by the curve of the graph starting to level out. Catalase is an enzyme found in food such as potato and liver. Its functions include the conversion of Hydrogen Peroxide, a powerful and potentially… 775 Words 4 Pages Determining the Effect of pH on Enzyme Activity Biology 1630 By: Princetta Samuels November 30, 2015 Abstract: The experiment was to assay and study an enzyme called peroxidase using a spectrophotometer. The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed down each time, if the bung is pushed down further then the volume in the tube will be less so the 30cm3 of gas is reached faster. Meaning that a lower pH level results in a higher enzyme activity reaction and a higher pH level results in a lower enzyme activity reaction Christianson, 2011. Enzyme function may be affected by many factors, including pH, temperature and the presence of chemical inhibitors. Increasing the Hydrogen Peroxide Concentration after the point of saturation has been reached will not cause the rate of reaction to go up any more.
Wash out the test tube twice to make sure that all of the previous reactants are gone. The three aspects of this scientific breakthrough are the fuel cells, hydrogen production, and hydrogen storage. The rate steadily increases when more substrate is added because more of the active sites of the enzyme are being used which results in more reactions so the required amount of oxygen is made more quickly. Catalase is one of the most vigorous enzymes known, and the rush of oxygen to the surface … of the liquid is spectacular. Then gradually rose to a 0. Catalase aids the decomposition of Hydrogen Peroxide the by products of which are water and oxygen as shown in the equations below.