Near the end of the lab, be prepared to show examples of each phenomenon on your computer screen. Most frog legs these days come from frog farms in Thailand and Indonesia. Holding the upper part of the frog firmly in one hand pull the skin down off of the leg. By studying genetics and inheritance, plants and animals have progressed biologically which means they are more productive and in cases, have more nutrients. To facilitate this, add comments to the pages of computer data you record. We used the program LabScribe to collect the data from the graph, and we also used the stimulator to stimulate the muscle through computer.
Use single shocks to watch the leg twitch. Trigeminal Pons Receives sensation from the face and innervates the muscles of mastication. The absolute force that a muscle can generate is dependent on the total number of muscle fibers. The muscle cell or fiber is the basic unit of a muscle. It is also important to have a general understanding of them because they will need to be used in calculations for the lab. So why not dissect a frog to find out how.
The object of the pithing operation is to destroy specified parts of the central nervous system. The acetylcholine is rapidly hydrolyzed by acetylcholine esterase on the skeletal muscle membrane in this region and thus does not accumulate in the junctional cleft. Extension, Flexion, Hip 997 Words 5 Pages 2: Physiology Lab Skeletal Muscle Physiology Student Name: Lab Summary Worksheet Directions: Read the following directions before starting the lab. Ciliary muscle, Cornea, Eye 1396 Words 5 Pages Lab 6 Biomechanics of Frog Skeletal Muscle I. Abdomen, Digestive system, Jaw 1141 Words 4 Pages Abstract: The purpose of this experiment was to examine how the compound action potentials propagate down the sciatic nerve of a frog based on varying stimuli.
Several varying stimuli were used and recorded by a software package. The force transducer must be held with a micromanipulator, so you can move in millimeter increments to stretch the attached muscle. The dissection tray must be firmly clamped so it can withstand the upward or sideways force of the stretching muscle. Plug the iWorx power cord into the outlet 6. Equipment available same as for Frog Muscle Lab I Frog hindlimb from which you must dissect the gastrocnemius muscle and sciatic nerve PowerLab computers with Scope and Chart Programs PowerLab stimulators and pin electrodes MacLab force transducer converts tension to mV; you should calibrate yours during lab with clamps that weigh 41.
Attach the cable of the Displacement Transducer to the bottom channel 3 input plug 3. Purpose This exercise is designed to demonstrate some mechanical and physiological properties of skeletal muscle using the gastrocnemius muscle of a frog. Please make sure that your answers. There are a couple questions asking whether my different hypothesis' were refuted or supported. Osmosis is known as the movement of water in and out of a cell.
Experimentally, what is measured at different degrees of stretch is the total force during nerve stimulation and contraction and passive force in the absence of nerve stimulation. Links: frog legs are a delicacy of French and Chinese cuisine. The strength of a muscle contraction, therefore, can be increased in two ways: by increasing the number of active motor units termed recruitment and by stimulating existing active motor units more frequently. Your dissected hindlimb should look like this although the sciatic nerve is not visible here. Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio.
Separate the gastrocnemius muscle from adjacent tissue with a glass probe. This was one of my favorite labs from General Biology 1 because it was extremely interesting and unlike any lab I had ever done before. Use the 5v supply for the excitation voltage! This active force is derived from interactions of the actin and myosin protein filaments. We'll tie slip knots with the thread to attach to the transducer. These demonstrate some of the important functional characteristics of skeletal muscle. Bone, Joint, Joints 956 Words 5 Pages Background: As members of the class Amphibia, frogs may live some of their adult lives on land, but they must return to water to reproduce. The electric shock acts as an action potential by changing the membrane permeability allowing the sodium and potassium ions to pass through.
You will be instructed on the use and calibration of the force transducer. As adults, they are primarily land animals, however they must return to the water to mate. Pithing: Insert a 10 cm rigid probe tapered to a sharp point through the skin into the foramen magnum between the base of the skull and the top of the vertebrae. Name each phase of a typical muscle twitch, and, on the following line, describe what is happening in each phase. What is the absolute minimum number of recording necessary to evaluate the hypothesis? Draw a diagram of the setup used in this lab exercise. Add the 100 mL cautiously to the 800 mL, stirring vigorously.